メニュー
| 日時 | 2022.12.9（17：00～18：00） |
|---|---|
| 会場 |
ミーティングID：854 0616 0651 パスコード：174627 |
| Google Classroom コード | rpppov4 |
| 講演者 | Dr. Edouard Pesquet |
| 所属機関 | ストックホルム大学 （スウェーデン） |
| 講演タイトル | "Swedish-Japanese collaboration to unravel the function behind the differences in lignin chemistry between cell types" ＜要旨＞ The biopolymer lignin, deposited in the cell walls of vascular cells, is essential for plant long-distance water conduction, structural support and resistance to biotic and abiotic constraints. Independently of the plant species, the cell wall layers of each vascular cell type contain specific lignin chemistries differing in aromatic substitutions and aliphatic functions. Yet, both the spatial control and the biological function of this conserved and specific lignin chemistry for the cell wall layers of each vascular cell type remain unclear. This lack of clarity is due to our current methodology restricted to ground or ball-milled samples that averages out the spatial and developmental changes of each cell type. We thus optimized in situ quantitative imaging methods to determine changes in lignin structure, concentration and/or composition at subcellular levels in whole plant biopsies using direct methods such as Raman spectroscopy, UV fluorescence and histochemistry [1-4]. We combined functional genetics, using a collection of mutants in Arabidopsis and poplar, with in situ quantitative imaging to unravel the molecular mechanisms controlling the spatial accumulation of specific lignin chemistries in distinct cell types and its impact on each cellular function. We found that specific lignin chemistries accumulated dynamically during the maturation of each cell type and morphotype, changing the concentration and composition of their lignin [5]. We found that lignin spatial accumulation in each cell type and cell wall layer depended on different combination of phenol oxidizing enzymes exhibiting substrate specificities [6]. We lastly showed that modifying this specific lignin chemistry or its dynamics impaired the cell wall biomechanics of each cell type and morphotype, and consequently plant growth and its response to abiotic constraints [5-6]. Altogether, we show that lignin chemistry is differently controlled for each cell type and morphotype during their maturation to dynamically adjust their function in response to developmental and environmental constraints. References [1] Decou R, Serk H, Ménard D, Pesquet E. Methods Mol. Biol. 1544, 233-247, 2017. [2] Blaschek L, Champagne A, Dimotakis C, Nuoendagula, Decou R, Hishiyama S, Kratzer S, Kajita S, Pesquet E. Front Plant Sci. 11, 109, 2020. [3] Blaschek L, Nuoendagula, Bacsik Z, Kajita S, Pesquet E. ACS Sustainable Chem. Eng. 8, 4900–4909, 2020. [4] Yamamoto M, Blaschek L, Subbotina E, Kajita S, Pesquet E. ChemSusChem. 13, 4400-4408, 2020. [5] Ménard D, Blaschek L, Kriechbaum K, Lee CC, Serk H, Lyubartsev A, Bacsik Z, Bergström L, Mathew A, Kajita S, Pesquet E. Plant Cell, 2022, in press. [6] Blaschek L, Murozuka E, Serk H, Ménard D, Pesquet E. Plant Cell, 2022, in revision. |
| 言語 | 英語 |
| 対象 | どなたでも、ご参加いただけます。 |
| 共催 | グローバルイノベーション研究院 食料分野 梶田ユニット 卓越大学院プログラム |
| お問い合わせ窓口 | グローバルイノベーション研究院・農学研究院 梶田 真也 e-mail: kajita（ここに@ を入れてください）cc.tuat.ac.jp |
| 備考 | 本セミナーはZoomにてご参加いただけます。 |
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